Characterization of the Antioxidant Effects of γ-Oryzanol: Involvement of the Nrf2 Pathway

Figure 5

Oryzanol induces an increase in Nrf2 target gene protein levels. HEK-293 cells were pretreated with 5 μg/mL ORY for 24 h followed by 24 h of 100 μM H₂O₂. Cells were then processed for measuring Nrf2 (a), HO-1 (b), GSS (c), and NQO1 (d) protein levels by Western blotting. Tubulin and actin were used as loading control according with the molecular weight of the above investigated proteins. Data are shown as mean ± SD; and versus untreated cells, Dunnett’s multiple comparison test.

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