Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 6

Research Article

Honokiol Ameliorates Myocardial Ischemia/Reperfusion Injury in Type 1 Diabetic Rats by Reducing Oxidative Stress and Apoptosis through Activating the SIRT1-Nrf2 Signaling Pathway

Figure 6

SIRT1 siRNA and Nrf2 siRNA transfection blunted HKL-induced antiapoptotic effects against SIR injury in HG-treated H9c2 cells. (a) Cellular morphology (100x). (b) Cellular viability. (c) Representative images of TUNEL staining (200x, bar = 200 μm). The apoptotic cells were detected by TUNEL (green), and the nuclei were detected by DAPI (blue). (d) Cellular apoptotic index. (e) Representative blots. (f) Bcl-2/Bax ratio. (g) Cleaved caspase 3 expression. (h) Cytosolic cytochrome c expression. Data are presented as the mean ± SEM ( in each group). versus the HG group, versus the HG + SIR group, versus the HG + SIR + HKL group.

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