Research Article
Honokiol Ameliorates Myocardial Ischemia/Reperfusion Injury in Type 1 Diabetic Rats by Reducing Oxidative Stress and Apoptosis through Activating the SIRT1-Nrf2 Signaling Pathway
Figure 6
SIRT1 siRNA and Nrf2 siRNA transfection blunted HKL-induced antiapoptotic effects against SIR injury in HG-treated H9c2 cells. (a) Cellular morphology (100x). (b) Cellular viability. (c) Representative images of TUNEL staining (200x, bar = 200 μm). The apoptotic cells were detected by TUNEL (green), and the nuclei were detected by DAPI (blue). (d) Cellular apoptotic index. (e) Representative blots. (f) Bcl-2/Bax ratio. (g) Cleaved caspase 3 expression. (h) Cytosolic cytochrome c expression. Data are presented as the mean ± SEM ( in each group). versus the HG group, versus the HG + SIR group, versus the HG + SIR + HKL group.
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |