Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 7

Research Article

Honokiol Ameliorates Myocardial Ischemia/Reperfusion Injury in Type 1 Diabetic Rats by Reducing Oxidative Stress and Apoptosis through Activating the SIRT1-Nrf2 Signaling Pathway

Figure 7

SIRT1 siRNA and Nrf2 siRNA transfection inhibited HKL-induced suppression on oxidative stress level against SIR injury in HG-treated H9c2 cells. (a) Intracellular SOD activity. (b) Intracellular MDA content. (c) Representative blots. (d) NQO-1 expression. (e) HO-1 expression. (f) gp91phox expression. (g) Representative images of DCFH-DA staining (300x, bar = 100 μm). (h) DCFH-DA intensity. Data are presented as the mean ± SEM ( in each group). versus the HG group, versus the HG + SIR group, versus the HG + SIR + HKL group.

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