Nicotine and Cotinine Inhibit Catalase and Glutathione Reductase Activity Contributing to the Impaired Osteogenesis of SCP-1 Cells Exposed to Cigarette Smoke

Figure 6

CSE generated an imbalance in the antioxidative system. Enzyme activities SOD (a), catalase (b), GPx (c), and GR (d) were determined with and without exposure to 5% CSE, 50 ng/ml or 320 ng/ml nicotine, and 100 ng/ml or 300 ng/ml cotinine. The enzymatic activity was expressed as the fold of control. Each experiment was conducted at least three times independently with triplicate. The statistical significance was determined by the Kruskal-Wallis H test followed by Dunn’s posttest. Data are represented as the mean ± SEM, and the significance was represented as , , and vs the control group.

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