Hydrogen Sulfide Alleviates Acute Myocardial Ischemia Injury by Modulating Autophagy and Inflammation Response under Oxidative Stress

Figure 6

NaHS treatment reduced autophagy and apoptosis induced by glucose deprivation in SOD1-knockdown neonatal rat cardiomyocytes. (a) Quantitative analysis of superoxide levels in SOD1-knockdown NRCMs under normal conditions or GD with or without 50 μM NaHS treatment. (b) Quantitative analysis of apoptosis levels in SOD1-knockdown NRCMs with or without NaHS treatment for 24 h. (c) Representative blots and quantitative analysis of LC3II/LC3I and S6K phosphorylation and AMPK phosphorylation after SOD1 knockdown in NRCMs with or without NaHS treatment. (d) Representative images and quantitative analysis of autophagy flux examined by fluorescent mCherry-GFP-LC3 signal (original magnification, ×400). Yellow puncta represent autophagosomes. Free red puncta represent autolysosomes. Values represent of three independent experiments. , , and .

(a)

(b)

(c)

(d)