Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus-Derived Mesenchymal Stem Cells

Figure 2

Cell proliferation and cell viability of NPMSC measuring by CCK-8. (a) NPMSCs were treated with different concentrations (0, 6.25, 12.5, 25, 50, 100 μM) of ICA for indicated times (1, 3, 5, 7 days). (b) Different concentrations (0, 6.25, 12.5, 25, 50, 100 μM) of ICA was added to the conventional cryoprotectant to verify the cryoprotective effects of ICA. NS means no statistical significant difference. The data are expressed as mean ± SD from three independent experiments. ( versus control; , , versus 0 μM of ICA).

(a)

(b)