Multifunctional Phytocompounds in Cotoneaster Fruits: Phytochemical Profiling, Cellular Safety, Anti-Inflammatory and Antioxidant Effects in Chemical and Human Plasma Models In Vitro

Table 3

UHPLC-PDA-ESI-MS³ data of polyphenols identified in the polar extracts from Cotoneaster fruits.

Number	Compounds		UV	(M-H)^−m/z	MS/MS m/z (% base peak)	CL	CDV	CHR	CN	CH	CDL	CS	CB	CZ
						%^b
1	Vanillic acid-hexoside	3.5	250, 290	329	MS^2: 167 (100); 123 (2); 107 (4)	3.8	3.1	3.4	3.4	2.5	1.4	2.3	0.9	1.7
2	Unidentified	4.4	250, 295	255	MS^2: 165 (23)	33.9	3.1	2.1	16.1	5.7	5.0	2.6	3.1	3.2
3	3-O-Caffeoylquinic acid	6.0	294, 325	353	MS^2: 191 (100); 179 (47); 135 (6)	1.8	10.4	5.3	15.1	5.1	5.1	8.1	5.7	1.9
4	3-O-p-Coumaroylquinic acid	9.4	285, 310	337	MS^2: 163 (100); 119 (10)	2.1	5.0	4.0	2.3	1.9	2.4	3.7	3.3	2.5
5	Caffeic acid hexoside	9.8	290, 323	341	MS^2: 179 (100); 135 (10)	2.3	8.2	2.7	5.1	1.2	0.6	2.5	1.0	2.8
6	Unidentified	10.0	285, 323	439	MS^2: 391 (100); 338 (17); 243 (10); 195 (55)	3.9	1.9	4.3	4.8	2.3	1.2	2.0	1.3	1.3
7	5-O-Caffeoylquinic acid (chlorogenic acid)^a	10.4	294, 325	353	MS^2: 191 (100); 179 (6)	29.4	28.3	29.5	26.0	23.5	23.0	17.9	17.3	10.8
8	4-O-Caffeoylquinic acid	10.9	294, 325	353	MS^2: 191 (21); 179 (47); 173 (100)	1.1	4.1	2.4	5.1	1.0	1.4	2.4	2.1	2.0
9	Procyanidin B-type dimer	13.7	280	577	MS^2: 451 (30); 425 (100); 407 (55); 289 (10); MS^3 (425): 407 (80); 273 (13)	0.7	1.6	0.5	1.2	1.1	1.2	0.9	nd	0.7
10	Procyanidin B-2^a	14.9	280	577	MS^2: 451 (25); 425 (100); 407 (62); 289 (14); MS^3 (425): 407 (95); 273 (9)	0.3	2.5	4.6	0.8	8.0	6.2	5.5	9.3	10.2
11	5-O-p-Coumaroylquinic acid	15.7	285, 310	337	MS^2: 191 (100); 163 (7)	11.7	3.1	1.2	3.6	1.2	0.6	0.8	1.4	1.9
12	(−)-Epicatechin^a	16.4	280	289	MS^2: 245 (100); 205 (28)	2.4	4.4	8.5	2.1	15.8	12.3	9.7	18.6	34.4
13	Procyanidin B-type dimer	17.3	280	577	MS^2: 451 (25); 425 (100); 407 (45); 289 (6); MS^3 (425): 407 (75); 273 (9)	nd	nd	nd	nd	0.4	nd	0.6	0.9	1.2
14	Procyanidin B-type tetramer	18.3	280	1153	MS^2: 1027 (15); 863 (80); 739 (15); 501 (05); 491 (58); 289 (100)	nd	nd	nd	nd	1.0	nd	0.6	1.1	1.4
15	Procyanidin C-1^a	20.6	280	865	MS^2: 847 (19); 739 (77); 713 (51); 695 (100); 577 (26); MS^3 (713): 695 (100); 561 (30); 543 (31); 425 (32); 407 (36)	0.5	1.9	3.2	nd	5.3	3.7	3.3	5.6	7.3
16	Procyanidin B-type tetramer	23.3	280	1153	MS^2: 863 (90); 739 (10); 501 (65); 491 (62); 289 (100)	nd	nd	2.1	nd	2.7	2.2	2.0	2.7	3.5
17	Quercetin 3-O-β-D-(2-O-β-D-xylosyl)galactoside^a	23.9	268, 355	595	MS^2: 463 (10); 445 (14); 300 (85); MS^3 (463): 343 (62); 301 (100)	nd	nd	4.0	nd	2.0	2.6	16.1	5.9	nd
18	Epicatechin derivative	26.2	280	739	MS^2: 587 (100); 451 (19); 339 (40); 289 (35)	nd	2.2	2.0	nd	1.1	nd	1.5	1.2	nd
19	Unidentified	26.3	280	451	MS^2: 341 (100); 217 (8)	nd	2.5	2.6	2.4	1.5	2.0	2.0	1.2	1.2
20	Quercetin rhamnoside-hexoside	26.7	275, 350	609	MS^2: 301 (100)	0.6	0.4	1.4	nd	0.7	3.2	2.3	0.9	1.8
21	Quercetin 3-O-β-D-galactoside (hyperoside)^a	27.1	265, 355	463	MS^2: 301 (100)	2.5	5.0	4.9	5.5	5.5	9.5	5.2	6.6	2.4
22	Querectin 3-O-β-D-(6-O-α-L-Rhamnosyl)glucoside (rutin)^a	27.3	260, 355	609	MS^2: 301 (100)	0.8	2.5	2.6	2.8	3.8	2.5	2.0	nd	2.2
23	Quercetin 3-O-β-D-glucoside (isoquercitrin)^a	28.0	265, 355	463	MS^2: 301 (100)	1.6	3.1	2.4	2.4	3.5	2.5	3.3	2.6	3.2
24	Procyanidin B-type dimer	28.6	280	577	MS^2: 425 (100); 407 (52); 289 (18)	0.6	1.6	2.0	1.0	1.6	2.0	1.6	2.0	2.4
25	Quercetin rhamnoside-hexoside	31.3	276, 350	609	MS^2: 301 (100)	nd	2.2	2.2	nd	0.4	4.1	nd	2.9	nd
26	Quercetin 3-O-α-L-rhamnoside (quercitrin)^a	32.4	276, 350	447	MS^2: 301 (100)	nd	2.8	2.2	nd	1.4	5.1	1.8	2.6	nd

^aIdentified with the corresponding standards; ^brelative contribution based on peak area on the UHPLC chromatograms ( = 280 nm) recorded at the extract concentration of 10 mg/mL and injection volume of 3 μL; nd—not detected; the values are means (); with RSD ≤ 5%. CL, C. lucidus; CDV, C. divaricatus; CHR, C. horizontalis; CN, C. nanshan; CH, C. hjelmqvistii; CDL, C. dielsianus; CS, C. splendens; CB, C. bullatus; CZ, C. zabelii.