Figure 6: GTs suppressed disturbed flow-induced inflammatory response in ECs. Human umbilical vein endothelial cells (HUVECs) cultured on μ-slides were exposed to laminar shear stress (LSS, 12 dyn/cm2), oscillatory shear stress (OSS, ±5 dyn/cm2), or static control for 24 h in the presence of GTs (500 μg/ml) or DMSO vehicle control (Veh). mRNA from treated cells was isolated and examined for the expression levels of proinflammatory genes (a) Vcam-1, (b) Tnfα, or (c) Il-6 using quantitative RT-PCR. Data are shown as mean ± SEM of triplicate experiments. Statistical significance was calculated using two-way ANOVA and post hoc Tukey’s tests, .