Dysfunction of Nrf2-ARE Signaling Pathway: Potential Pathogenesis in the Development of Neurocognitive Impairment in Patients with Moderate to Severe Obstructive Sleep Apnea-Hypopnea Syndrome

Figure 2

Nrf2 and HO-1 mRNA and protein expression in PBMCs of the OSAHS and control groups. (a) Relative mRNA expression of Nrf2 and HO-1 was analyzed by quantitative real-time PCR; normalized gene expression was obtained using the 2-ΔCt method. Data are expressed as mean ± SD; 0.01. (b-c) Relative protein expression of Nrf2 and HO-1 was analyzed using Western blot. Normalized protein expression was obtained as the ratio of optical density between target protein and β-actin. Data are expressed as mean ± SD. (d) The average relative quantification obtained by densitometric analysis of all the samples. . 1—OSAHS group; 2—healthy control group.

(a)

(b)

(c)

(d)