A Novel Mechanism of Mesenchymal Stromal Cell-Mediated Protection against Sepsis: Restricting Inflammasome Activation in Macrophages by Increasing Mitophagy and Decreasing Mitochondrial ROS

Figure 2

Treatment with BMSCs reduced the levels of sepsis-induced inflammation. (a-b) Treatment with BMSCs attenuated sepsis-induced inflammatory cell infiltration in organs. Inflammatory cell infiltration was determined by immunohistochemistry staining. Red arrows indicate inflammatory cells. (a) The sections were immunohistochemically analyzed using antibodies against Mac-3 for macrophages (red arrows). Scale bar, 100 μm. (b) The sections were immunohistochemically analyzed using antibodies against Ly6G for neutrophils (red arrows). Scale bar, 100 μm. (c) Quantitative analysis of positive cell numbers per five different HPFs (high magnification fields). (d) Levels of the proinflammatory cytokines IL-6, IL-1β, and TNF-α and anti-inflammatory cytokine IL-10 in serum were measured by ELISA. Error bars represent the means ± s.e.m. per group. and . Data are representative of three independent experiments.

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