Tanshinone IIA Pretreatment Protects H9c2 Cells against Anoxia/Reoxygenation Injury: Involvement of the Translocation of Bcl-2 to Mitochondria Mediated by 14-3-3η

Figure 2

Low expression of 14-3-3η abolished the protective effect of Tanshinone IIA (TSN) preconditioning on lactate dehydrogenase (LDH) activity and cell viability in H9c2 cells subjected to anoxia/reoxygenation (A/R) injury. (a, b) Effects of TSN on the expression of 14-3-3η in H9c2 cells subjected to A/R injury by Western blot analysis. Untreated cells which served as the AD14-3-3η RNAi group were incubated with adenovirus containing 14-3-3ηsiRNA, and the AD-scrRNA group was incubated with empty adenovirus vector. β-Actin served as a loading control (a). Pretreatment with TSN increased 14-3-3η expression under A/R injury, which was silenced by AD14-3-3η RNAi (b). TSN pretreatment inhibited activity of LDH (c) and increased cell viability (d) under A/R injury, and this effect was silenced by AD14-3-3η RNAi. Values are presented as the for five individual experiments. (A) versus control group; (B) versus A/R group; (C) versus TSN + A/R group; (D) versus A/R group; (E) versus AD14-3-3ηRNAi group; (F) versus AD14-3-3ηRNAi group.

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