Tanshinone IIA Pretreatment Protects H9c2 Cells against Anoxia/Reoxygenation Injury: Involvement of the Translocation of Bcl-2 to Mitochondria Mediated by 14-3-3η

Figure 3

Protein interaction among Bcl-2, 14-3-3η, and VDAC-1 on Tanshinone IIA (TSN) preconditioning in H9c2 cells subjected to A/R injury. (a) Cells were pretreated with TSN for 2 days prior to A/R injury and extraction of proteins. Proteins were immunoprecipitated with anti-14-3-3η, anti-Bcl-2, and anti-VDAC-1, respectively, and immunoblotted with anti-Bcl-2 and anti-VDAC-1. (b) Cells were pretreated with TSN for 2 days prior to A/R injury. The cyan color reflects overlap of VDAC-1 and 14-3-3η. A yellow color reflects overlap of VDAC-1 and Bcl-2, a purple color reflects overlap of 14-3-3η and Bcl-2, and a white color reflects overlap of 14-3-3η, Bcl-2, and VDAC-1. Data were collected from three individual experiments.

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