Tanshinone IIA Pretreatment Protects H9c2 Cells against Anoxia/Reoxygenation Injury: Involvement of the Translocation of Bcl-2 to Mitochondria Mediated by 14-3-3η

Figure 6

Inhibiting mitochondrial translocation of Bcl-2 by silencing 14-3-3η expression abolished the protective effect of Tanshinone IIA (TSN) pretreatment on mPTP opening and cyt c release in H9c2 cells subjected to anoxia/reoxygenation (A/R) injury. Ca²⁺-induced swelling of mitochondria was used to access mPTP opening. mPTP opening led to mitochondrial swelling and caused a change in absorbance at 520 nm. (a) The changes in absorbance were detected every 2 min to access the progress of mPTP opening. (b) The extent of mPTP opening was accessed by using the equation . (c, d) The cyt c release from mitochondria to the cytoplasm was accessed by Western blot analysis. Values are presented as the for five individual experiments. (A) versus control group; (B) versus A/R group; (C) versus A/R group; (D) versus TSN + A/R group; (E) versus TSN + A/R group; (E) versus TSN + A/R group; (F) versus AD14-3-3ηRNAi group.

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