Gastrodin Protects Cardiomyocytes from Anoxia/Reoxygenation Injury by 14-3-3η

Figure 5

The effect of gastrodin (GAS) on the expression of 14-3-3η in H9c2 cells exposed to anoxia/reoxygenation (A/R) injury. 14-3-3η expression was evaluated by Western blot, and β-actin was used as an internal control. (a) The expression of the 14-3-3η protein significantly increased in the A/R group ( versus control group). In the concentration-effect GAS + A/R group, the expression of 14-3-3η in the GAS + A/R group was higher than that in the A/R group ( and versus A/R group), and the 20 mg/L GAS + A/R group presented with the highest expression of 14-3-3η ( versus 5, 10, 40, 80, 160, and 320 mg/L GAS + A/R group). (b) The expression of the 14-3-3η protein significantly increased in the A/R group ( versus control group). And the expression of the 14-3-3η protein significantly increased in both the GAS + A/R group and the NC + GAS + A/R group ( versus A/R group) but decreased in the siRNA14-3-3η + GAS + A/R group ( versus GAS + A/R group). Data are expressed as the mean ± SEM ().

(a)

(b)