Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation

Figure 3

qPCR studies for kidney biopsies taken prior to transplant. For a separate series of kidney transplants, biopsies were collected for qPCR. (a) PCR primers used for qPCR. qPCR reactions were performed in a final volume of 20 μl that contained 15 μl of Fast SYBR© Green Master Mix (Life Technologies), 1000 nM primer (ALDH2, ALDH7A1 or GAPDH) or 500 nM primer (ALDH4A1), and 10 ng cDNA. (b–e) qPCR results for (b) ALDH2, (c) ALDH7A1, (d) ALDH4A1, and (e) ALDH1A1. ALDH2, ALDH7A1, and ALDH4A1 by qPCR were significantly elevated in kidneys that did not develop delayed graft function versus those kidneys that developed delayed graft function (/group, ). Ct values of each gene were normalized to GAPDH to calculate the delta Ct value. (-) = donor kidneys without delayed graft function; (+) = donor kidneys with delayed graft function.

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