Isolation and Characterization of NP-POL Nonapeptide for Possible Therapeutic Use in Parkinson’s Disease

Figure 3

The effects of NP-POL on 6-OHDA-induced cytotoxicity of PC12 cells. PC12 cells (1 × 10⁴/well) were incubated for 24 h with inducers: NP-POL (1 μg/ml–150 μg/ml) (a) or toxin 6-OHDA (1 μM–200 μM) (b). To measure the neuroprotective effect of NP-POL, the nonapeptide was preincubated with cells for 1 h before application of 150 μM 6-OHDA (c) or was applied simultaneously with 6-OHDA (150 μM) (d). Cell viability was measured by the MTT assay. Data are presented as mean ± SD (). Results were considered significant at versus untreated cells and at , , and versus 6-OHDA.

(a)

(b)

(c)

(d)