Research Article
Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion
Figure 1
CSE is markedly upregulated in atheromatous lesions. (a, upper panels) Immunohistochemistry detection of the CSE expression in human carotid artery specimens. Macroscopic pictures, H&E, and CSE staining are shown. Magnification of the human histology samples was 150x. Arrows indicate endothelial cells, crosses show smooth muscle cells, and comparison signs mark macrophages. (a, lower panels) Pieces of carotid artery representing the healthy vessel; atheromatous and complicated lesions were selected by macroscopic examination. Representative Western blot showing CSE expression in carotid artery tissue lysates (20 μg/lane) (, three human carotid artery specimens from three patients in each group). Immunoblots were reprobed with GAPDH. The bar graph shows CSE expression normalized to GAPDH. and compared to healthy samples. (b) ApoE−/− mice were kept on normal chow diet or atherogenic diet for 8 weeks. (b, upper panels) H&E and CSE staining are shown. Magnification of the mice aortas was 700x. Arrows indicate endothelial cells, crosses show smooth muscle cells, and comparison signs mark macrophages. (b, lower panels) Representative Western blot showing CSE expressions of carotid artery samples (10 μg/lane) (, each performed in triplicate). Immunoblots were reprobed with GAPDH. The bar graph shows CSE expression normalized to GAPDH. (c) Endogenous H2S levels in different types of the carotid arteries ().
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