Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion

Figure 5

Sulfide inhibits the formation of covalently cross-linked Hb multimers. (a) Hb (5 μmol/L) was incubated with H₂O₂ (75 μmol/L) in the presence or absence of the H₂S donors NaSH, GYY4137, AP67, and AP72 (200 μmol/L) at 37°C for 90 minutes. Samples (0.5 μg Hb) were subjected to SDS-PAGE and Hb species were detected by Western blotting. Representative experiment, . (b and d) Quantification of the multimer formation was showed. (c) Hb (5 μmol/L) was incubated with oxLDL (50 μg/ml) in the presence or absence of the sulfide donors NaSH, GYY4137, AP67, and AP72 (200 μmol/L) at 37°C for 4 hours. Samples (0.5 μg Hb) were subjected to SDS-PAGE and Hb species were detected by Western blotting. Representative experiment, . indicates statistical significance ().