Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells

Figure 5

PSD-A induces apoptosis and ER stress via JNK activation. (a) A549 cells were treated with 100 nM PSD-A in the presence or absence of NAC (3 mM), BAPTA-AM (10 μM), SB203580 (5 μM), and SP600125 (20 μM) for 24 h, and apoptosis was determined using flow cytometry. (b) Data from (a) are expressed as mean ± SEM (). Columns not sharing the same superscript letters differ significantly (). (c) A549 cells were treated with PSD-A in a similar fashion as described above; total cell lysates were extracted and subjected to Western blot for the expression of cleaved PARP. (d) A549 cells were treated with 50 nM PSD-A in the presence or absence of various inhibitors as indicated for 8 h. The expressions of p-JNK(1/2), p-p38, and p-eIF2α were measured by Western blot.

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