Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells

Figure 7

Computational modeling of PSD-A binding with STAT3 and effect of STAT3 knockdown on cell growth and death. (a) The docking simulation results obtained from PyMOL showing hydrogen bonding between PSD-A and STAT3 SH2 domain. (b) Two-dimensional representation of these interactions between PSD-A and STAT3, plotted by LIGPLOT. Hydrogen bond interactions are indicated as green dotted lines. (c) Western blot expression of STAT3 in scrambled (Sc) treated and STAT3 knockdown A549 cells. (d) Evaluation of the effect of STAT3 knockdown on proliferation of A549 cells by MTT assay. (e) Effect of STAT3 knockdown on PSD-A-induced cell death in A549 cells was determined by TBE assay. Data are expressed as mean ± SEM of 3 independent experiments. Statistical significance between scrambled shRNA and STAT3 shRNA was determined by two-way ANOVA followed by the Bonferroni post hoc test for multiple comparisons. .

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