Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 4

Research Article

ATM Induces Cell Death with Autophagy in Response to H2O2 Specifically in Caenorhabditis elegans Nondividing Cells

Figure 4

Caffeine prevents H2O2-dependent intestinal cell death. (a) Adult N2 and atm-1(tm5027) worms were pretreated with caffeine (20 mM caffeine for at least 6 hours). Then, the worms were treated with 88 mM H2O2 for 1 hour at 20°C. After the treatment, the worms were stained with AO for 5 minutes at 20°C and observed microscopically after destaining twice with M9 buffer and fixing with PFA. (b) The fraction of animals with somatic AO fluorescence. At least 150 animals were counted for each condition. All data are mean ± SD and means significantly different by a multiple-comparison one-way ANOVA (Tukey’s test) ().

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