ATM Induces Cell Death with Autophagy in Response to H₂O₂ Specifically in Caenorhabditis elegans Nondividing Cells

Figure 4

Caffeine prevents H₂O₂-dependent intestinal cell death. (a) Adult N2 and atm-1(tm5027) worms were pretreated with caffeine (20 mM caffeine for at least 6 hours). Then, the worms were treated with 88 mM H₂O₂ for 1 hour at 20°C. After the treatment, the worms were stained with AO for 5 minutes at 20°C and observed microscopically after destaining twice with M9 buffer and fixing with PFA. (b) The fraction of animals with somatic AO fluorescence. At least 150 animals were counted for each condition. All data are mean ± SD and means significantly different by a multiple-comparison one-way ANOVA (Tukey’s test) ().

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