Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 5

Research Article

ATM Induces Cell Death with Autophagy in Response to H2O2 Specifically in Caenorhabditis elegans Nondividing Cells

Figure 5

CeATM-1 induces cell death with autophagy in response to H2O2. (a) The synchronized adult MAH236 (lgg-1p::GFP::lgg-1 + odr-1p::RFP) and atm-1(tm5027/[lgg-1p::GFP::lgg-1 + odr-1p::RFP]) worms were pretreated with 20 mM caffeine for 6 hours. Then, the worms were treated with 0.25% MMS or 44 mM H2O2 for 7 hours at 20°C. After the treatments, the worms were fixed with PFA. After washing twice with M9 buffer, the worms were examined microscopically. (b) The fraction of animals with increased somatic GFP. At least 150 animals were counted for each condition. All data are mean ± SD and means significantly different by Student’s -test (). The photographs of a worm and a microscope were obtained from TogoTV (© 2016 DBCLS TogoTV).

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