Inhibitory Effects of Momordicine I on High-Glucose-Induced Cell Proliferation and Collagen Synthesis in Rat Cardiac Fibroblasts

Figure 1

Effects of momordicine I on high-glucose-induced cell proliferation and collagen synthesis in rat cardiac fibroblasts. (a) Chemical structure of momordicine I. (b) Effect of momordicine I (0.1, 0.3, 1, 3, and 10 μM) on cell viability in cardiac fibroblasts. Cell viability was quantified through MTT assay. Effects of momordicine I on high-glucose-stimulated fibroblast proliferation (c) and collagen synthesis (d) were assessed through BrdU and ³H-proline incorporation. Rat cardiac fibroblasts were cultured in a serum-free normal-glucose medium (5.6 mM glucose) or high-glucose medium (25 mM glucose) in the absence or presence of momordicine I (0.1, 0.3, and 1 μM) for 24 h. Results were presented as mean ± SEM (). versus the control group; versus the high-glucose group.

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