Inhibitory Effects of Momordicine I on High-Glucose-Induced Cell Proliferation and Collagen Synthesis in Rat Cardiac Fibroblasts

Figure 2

Effects of momordicine I on high-glucose-induced TGF-β1 secretion and Smad2/3 phosphorylation in rat cardiac fibroblasts. (a) Cardiac fibroblasts treated using a normal-glucose medium or high-glucose medium in the absence or the presence of momordicine I (0.1, 0.3, 1 μM) for 24 h and the secretion of TGF-β1 in cardiac fibroblast supernatants measured through ELISA. Results are presented as mean ± SEM (). (b) Effect of momordicine I on high-glucose- or TGF-β1-induced Smad2/3 phosphorylation. The protein expression levels of Smad2/3 and p-Smad2/3 were detected using Western blotting following incubation with momordicine I (1 μM) for 12 h; cardiac fibroblasts were exposed to high glucose for 24 h or TGF-β1 (10 ng/mL) for 2 h. Representative micrographs of Smad2/3 and p-Smad2/3 expression in Western blot analysis (upper) and quantitative results (lower). Results are presented as mean ± SEM (). versus the control group; versus the high-glucose group.

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