Inhibitory Effects of Momordicine I on High-Glucose-Induced Cell Proliferation and Collagen Synthesis in Rat Cardiac Fibroblasts

Figure 3

Momordicine I inhibits high-glucose-induced ROS in rat cardiac fibroblasts. (a) Effects of momordicine I on high-glucose-induced ROS generation. Cardiac fibroblasts were cultured in a normal-glucose medium or high-glucose medium for 30 min or preincubated with momordicine I (1 μM, for 12 h) or NAC (5 mM, for 30 min) and then stimulated with the high-glucose medium for 30 min. Column bar graph of mean cell fluorescence for DCF. The fluorescence intensities in the control cells are expressed as 100%. (b) Effects of momordicine I and NAC on the high-glucose-induced secretion of TGF-β1 in cardiac fibroblasts. Effects of momordicine I and NAC on high-glucose-induced fibroblast proliferation (c) and collagen synthesis (d). Cardiac fibroblast cells were cultured in the control medium or high-glucose medium in the absence or presence of momordicine I (1 μM) or NAC (5 mM) for 24 h. Results are presented as mean ± SEM (). versus the control group; versus the high-glucose group.

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