Effect of the Antioxidant Lipoic Acid in Aortic Phenotype in a Marfan Syndrome Mouse Model

Figure 4

Quantitative analysis of microfluorotopography of DHE oxidation products in (a) 1-month-, (b) 3-month-, and (c) 6-month-old wild-type (WT) and Marfan syndrome (MFS) mice in the aortic arch and thoracic and abdominal aorta segments. (d) Representative photomicrographs of six-month-old wild-type (WT) and Marfan syndrome (MFS) mice, showing microfluorotopography of DHE oxidation products. Red staining indicates the fluorescence signal by DHE under 400x magnification. Bars: 50 μm. (e) Sections incubated with PEG-SOD showing no reactivity. (f) Representative photomicrographs of six-month-old wild-type (WT) and Marfan syndrome (MFS) mice, showing DMPO-protein radical adduct detection in the aortic arch and thoracic and abdominal aorta segments. Green staining indicates the fluorescent signal by DMPO, and blue stain (DAPI) indicates the nuclei under 400x magnification. Bars: 50 μm. (g) Quantitative analysis of DMPO-protein radical adduct detection in 6-month-old wild-type (WT) and Marfan syndrome (MFS) mice in the aortic arch and thoracic and abdominal aorta segments. ^a versus WT1; ^b versus MFS1; ^c versus WT1; ^d versus WT3; ^e versus MFS3; ^f versus WT6; ^g versus MFS1; ^h versus WT3; ⁱ versus WT6. Data expressed in mean ± SEM.

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