Dexmedetomidine Ameliorates Acute Stress-Induced Kidney Injury by Attenuating Oxidative Stress and Apoptosis through Inhibition of the ROS/JNK Signaling Pathway

Figure 5

DEX improved the apoptosis of rat renal tubular cells induced by acute stress. (a) The apoptosis of renal tissue was measured by the TUNEL Apoptosis kit, in which green cells represent TUNEL-positive apoptotic cells. TUNEL (green) detects apoptosis, DAPI (blue) locates the nucleus, and MERGE is the combination of TUNEL and DAPI. All fluorescence images were observed with a fluorescence microscope at a magnification of 200. Quantitative results of TUNEL analysis. Immunohistochemistry of cleaved caspase 3 protein: (b) C group, (c) AS group, and (d) D + AS group; bars = 50 μm. Data are expressed as mean ± SEM (). versus C group. versus AS group.

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