SGK1 Inhibits Autophagy in Murine Muscle Tissue

Figure 1

LC3-II and ATG12-ATG5 abundances are significantly increased in the red muscle tissue of Sgk1 knockout mice. LC3 lipidation was analysed by Western blotting (upper panel) and quantified (lower panels) in protein extracts from the red muscle of Sgk1 knockout (Sgk1−/−) and wild-type (Sgk1+/+) mice () (a). Of those, mice were either fed (F, ) or starved (S, ) as indicated (sample ID). LC3-positive control (Ctrl) was used to indicate the migration of nonlipidated LC3-I and lipidated LC3-II. Red lines on the box plots (lower panel, left) represent the mean values of all 6 Sgk1 knockout (Sgk1−/−) and wild-type (Sgk1+/+) mice (lower panel, left) or with regard to fed and starved conditions (lower panel, right). values are provided. In parallel, the abundance of the ATG12–ATG5 (ATG12–5) conjugate was likewise assessed (b). Supporting data is provided (Suppl. Data Set).

(a)

(b)