Research Article
SGK1 Inhibits Autophagy in Murine Muscle Tissue
Figure 5
SGK1 silencing provokes an increase of GFP-WIPI1 puncta. Stable GFP-WIPI1 expressing U-2 OS cells were transiently transfected with control siRNA (siControl) or siSGK1 and fed or starved for 3 h with or without bafilomycin A1 (BafA1). (a) Representative images for starved conditions with and without BafA1 treatment are shown. Scale bars 40 μm. (b) The percentage GFP-WIPI puncta cells were calculated (up to 1541 cells per condition, ). values are provided. Supporting data is provided (Suppl. Data Set).
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