Chlorophyll-Mediated Changes in the Redox Status of Pancreatic Cancer Cells Are Associated with Its Anticancer Effects

Figure 7

The effect of chlorophylls on the AKT and ERK activation in PaTu-8902 human pancreatic cancer cells. PaTu-8902 pancreatic cancer cells grown in duplicate were incubated in the absence (control) or in the presence of either chlorophyll a or chlorophyllin for 1 h. Cell lysates were probed with phospho-Akt (Ser473) and phospho-ERK antibodies to determine the phosphorylation level of AKT and ERK2, respectively. Immunoblotting with ERK1/2 and p120 RasGAP antibodies was used to confirm equal protein loading.