Oxidative Stress-Mediated Overexpression of Uracil DNA Glycosylase in Leishmania donovani Confers Tolerance against Antileishmanial Drugs

Figure 5

Effect of methotrexate on wild-type (WT) and UNG-OE L. donovani strains. (a) Total DNA isolated from WT and UNG-OE cells that were treated with 250 μM methotrexate for 7 hours (lane 2) and 24 hours (lane 4). Lanes 1 and 3 correspond to untreated cells processed identically. Isolated DNA samples were analysed on 1% agarose gel stained with ethidium bromide, and the inverted picture was presented. (b) The inhibitory concentrations for MTX were determined for both WT and UNG-OE cells. Untreated parasites were used as control in this experiment. . ns, nonsignificant. For the meaning of these statistical symbols, refer to ligand of Figure 1. The analysis was performed by Student’s t-tests using GraphPad Prism 5.0.

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