Oxidative Stress-Mediated Overexpression of Uracil DNA Glycosylase in Leishmania donovani Confers Tolerance against Antileishmanial Drugs

Figure 9

Effect of UNG overexpression on infectivity assessed by ex vivo infection in murine macrophages. (a) Microscopic pictures of Giemsa-stained uninfected macrophages (i), macrophages infected with WT (ii), and UNG-OE (iii), respectively. (b) Percentage of infected macrophages determined at 12 and 24 hours postinfection. (c) Number of amastigotes per 100 infected macrophages recorded at 12 and 24 hours postinfection. The results are expressed as mean ± SD of three independent experiments. Data was analysed using mix model ANOVA, followed by Bonferroni posttests using GraphPad Prism 5.0. Mixed model ANOVA determines how percentage of infected macrophages/number of amastigotes per 100 macrophages was affected by different factors (L. donovani strains, WT or UNG-OE, and different time intervals of 12 and 24 hours postinfection). The y-axis in two bar graphs corresponds to percentage of infected macrophages/number of amastigotes per 100 macrophages of WT/UNG-OE leishmania cells. , , and . ns, nonsignificant. For the meaning of these statistical symbols, refer to ligand of Figure 1.

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