Sulforaphane Modulates AQP8-Linked Redox Signalling in Leukemia Cells

Figure 2

Effect of SFN on AQP8 expression in B1647 cell line. B1647 cells were incubated for 24 h with different SFN concentrations and (a) RNA was extracted from the cells and samples subjected to RT-PCR analysis using specific primers as described in Materials and Methods section. Normalized expression levels were calculated relative to control cells according to the 2^-∆∆Cq method; (b) proteins were extracted, separated by SDS-PAGE, transferred to nitrocellulose membrane, and immunoassayed using anti-AQP8 and anti-β-actin antibodies as reported in Materials and Methods section. Immunoblot is the representative of three independent experiments, and densitometric analysis, normalized to β-actin, is expressed as fold decrease with respect to control. , significantly different from control cells.

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