Sulforaphane Modulates AQP8-Linked Redox Signalling in Leukemia Cells

Figure 5

Effect of SFN on Nox2 and Nox4 expression and phosphorylation level of VEGFR-2 and Akt in B1647 cell line. B1647 cells were incubated for 24 h with different SFN concentrations. At the end of incubation, cells were lysed, and proteins were separated by SDS-PAGE, transferred to nitrocellulose membrane, and immunoassayed using specific antibodies as reported in Materials and Methods section. Immunoblots are the representative of three independent experiments, and densitometric analysis, normalized to β-actin, is expressed as fold decrease with respect to control. , significantly different from control cells.