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Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 4237812, 8 pages
https://doi.org/10.1155/2018/4237812
Research Article

The Ratio of Plasma and Urinary 8-oxo-Gsn Could Be a Novel Evaluation Index for Patients with Chronic Kidney Disease

1Department of Nephrology, Beijing Hospital, National Center of Gerontology, Beijing 100730, China
2The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing 100730, China
3School of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China
4National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing 100730, China
5Department of Anesthesiology, Beijing Hospital, National Center of Gerontology, Beijing 100730, China
6The Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan

Correspondence should be addressed to Jian-Ping Cai; moc.anis.piv@16pjiac

Received 5 September 2017; Accepted 19 October 2017; Published 17 January 2018

Academic Editor: Ada Popolo

Copyright © 2018 Yong-Hui Mao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nucleic acid oxidation plays an important role in the pathophysiology progress of a variety of diseases. 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn), which originate from DNA and RNA oxidation, were the most widely used indicators for oxidative stress. The study investigated the relation between 8-oxo-dGsn, 8-oxo-Gsn, and CKD. 146 patients with CKD were divided into five disease stages, and their fasting blood and morning urine were collected. The levels of 8-oxo-dGsn and 8-oxo-Gsn in plasma and urine were quantified by LC-MS/MS. The ratio of urinary 8-oxo-Gsn to creatinine increased from stages 1 to 4 corresponding to the increased severity of CKD, but it decreased in stage 5. And plasma 8-oxo-Gsn gradually increased with the decline of renal function. In particular, the increased ratio of plasma and urine 8-oxo-Gsn in stage 5 exceeded the concentration of creatinine. This trend was similar to the estimated glomerular filtration rate (eGFR), which indicates that 8-oxo-Gsn could be an appropriate indicator for renal function. Our finding indicates that as the disease progresses, RNA oxidation is increased. The significant increase in the ratio of plasma and urinary 8-oxo-Gsn is a novel evaluation index of end-stage renal disease.