17β-Estradiol Regulates Microglia Activation and Polarization in the Hippocampus Following Global Cerebral Ischemia

Figure 10

Neurotoxicity of LPS-activated BV2 microglia cells is inhibited by E2 in vitro. BV2 cells were activated using LPS. Conditioned media from control, LPS-activated, and LPS + E2-treated cells were transferred to hippocampal neuronal cell line, HT-22 cells. HT-22 cells were treated with these conditioned media, supernatant was harvested for LDH assay, and cells were harvested for cleaved-caspase3 analysis. (a) LDH levels using the LDH assay kit were determined in the control, LPS, and LPS + E2-treated groups. This indicates that LPS-activated cells have increased LDH release, and this is suppressed by E2 treatment. (b, c) Western blot analysis and quantification from HT-22 cells indicate that LPS group had increased cleaved-caspase3 levels which are significantly suppressed by E2 treatment (n = 5-6 per group) (, control versus LPS, , LPS versus LPS + E2).

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