Research Article
17β-Estradiol Regulates Microglia Activation and Polarization in the Hippocampus Following Global Cerebral Ischemia
Figure 3
Estrogen suppresses gene expression of M1 markers and upregulates gene expression of M2 markers in the hippocampus after global cerebral ischemia. (a–c) mRNA samples from the hippocampus at days 3 and 7 were collected and analyzed for gene expression of M1, proinflammatory markers, TNF-α, CD68, and IL-1β. E2 treatment significantly suppressed gene expression of these markers. (d–f) mRNA samples from the hippocampus at days 3 and 7 were collected and analyzed for gene expression of M2, anti-inflammatory markers, Arginase1, CD206, and Ym1. E2 treatment significantly upregulated gene expression of these markers (n = 5-6 animals per group) (, GCI versus GCI + E2).
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |