Research Article
17β-Estradiol Regulates Microglia Activation and Polarization in the Hippocampus Following Global Cerebral Ischemia
Table 2
Primers used for RT-PCR analysis of in vitro samples.
| | Gene | Forward primer | Reverse primer |
| | CD86 | 5 GACCGTTGTGTGTGTTCTGG 3 | 5 GATGAGCAGCATCACAAGGA 3 | | iNOS | 5 CAAGCACCTTGGAAGAGGAG 3 | 5 AAGGCCAAACACAGCATACC 3 | | CD32 | 5 AATCCTGCCGTTCCTACTGATC 3 | 5 GTGTCACCGTGTCTTCCTTGAG 3 | | Arginase1 | 5 CAGAAGAATGGAAAGAGTCAG 3 | 5 CAGATATGCAGGGAGTCACC 3 | | CD206 | 5 CAAGGAAGGTTGGCATTTGT 3 | 5 CCTTTCAGTCCTTTGCAAGC 3 | | Ym1 | 5 CAGGGTAATGAGTGGGTTGG 3 | 5 CACGGCACCTCCTAAATTGT 3 | | IL-18 | 5 ACCAAGTTCTCTTCGTTGAC 3 | 5 TCACAGCCAGTCCTCTTAC 3 | | IL-1β | 5 TACTGAACTTCGGGGTGATTGGTCC 3 | 5 CAGCCTTGTCCCTTGAAGAGAACC 3 | | IL-12p35 | 5 CTCCTAAACCACCTCAGTTTGGCCAGGGTC 3 | 5 TAGATGCTACCAAGGCACAGGGTCATCATC 3 | | IL-4 | 5 AGATGGATGTGCCAAACGTCCTCA 3 | 5 GGATTATG ACTGCCACTGCGAC 3 | | IL-13 | 5 TGAGGAGCTGAGCAACATCACACA 3 | 5 TGCGGTTACAGAGGCCATGCAATA 3 | | IL-10 | 5 CCAAGCCTTATCGGAAATGA 3 | 5 TTTTCACAGGGGAGAAATCG 3 | | 18S | 5 AACCTGCTGGTGTGTGACGTTC 3 | 5 CAGCACGAGGCTTTTTTGTTGT 3 |
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