Induction of NLRP3 Inflammasome Activation by Heme in Human Endothelial Cells

<div>The coordinated iron is critical in heme-mediated induction of IL-1<i>β</i>. (a and b) HUVECs primed with LPS (10 <i>μ</i>g/mL, 24 h) were stimulated with heme, PPIX, or FeSO<sub>4</sub> (25 <i>μ</i>mol/L). (a) IL-1<i>β</i> mRNA level (4 h) was determined by quantitative RT-PCR. (b) Active IL-1<i>β</i> levels in cellular supernatants (24 h) were determined by ELISA. 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Induction of NLRP3 Inflammasome Activation by Heme in Human Endothelial Cells 