Induction of NLRP3 Inflammasome Activation by Heme in Human Endothelial Cells

<div>Oxidized Hb species increase ROS formation but fail to induce IL-1<i>β</i> production and maturation in HUVECs. (a–c) Naive (a–c) or LPS-primed (d) HUVECs were treated with heme (25 <i>μ</i>mol/L) Hb, metHb, or ferrylHb (25 or 250 <i>μ</i>mol/L). (a and b) Following the treatments (a: 25 <i>μ</i>mol/L and b: 250 <i>μ</i>mol/L) (4 h), ROS production was measured with DCFDA assay. (c and d) IL-1<i>β</i> mRNA level (4 h) was determined by quantitative RT-PCR. 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Oxidative Medicine and Cellular Longevity

fig8

Figure 8

Figure 8: Induction of NLRP3 Inflammasome Activation by Heme in Human Endothelial Cells 