Aucubin Protects against Myocardial Infarction-Induced Cardiac Remodeling via nNOS/NO-Regulated Oxidative Stress

Figure 5

Aucubin attenuates oxidative stress and increases thioredoxin (Trx) in vivo and vitro. (a) and (b) Western blot analysis of P67, gp91, SOD, and Trx in the indicated heart tissue post-MI ((a) representative blots and (b) statistical analysis result) (). (c) NO production in the indicated heart tissue. versus vehicle-sham and versus vehicle-MI. (d) and (e) Western blot analysis of P67, gp91, SOD, and Trx in the aucubin- (50 μM) pretreated H9c2 cardiomyocytes after exposure to hypoxia for 24 h ((a) representative blots and (e) statistical analysis result) ( sample). (f) ROS level detected by DCF-DA in the indicated group ( sample). (g) NO production in the indicated group ( sample). versus normoxia + PBS and versus hypoxia + PBS. All experiments were repeated independently three times.

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