2-Methoxyestradiol Attenuates Testosterone-Induced Benign Prostate Hyperplasia in Rats through Inhibition of HIF-1α/TGF-β/Smad2 Axis

Figure 5

(a) Western blot analysis of HIF-1α and p-Smad2 expression in prostate tissues. I: untreated control; II: testosterone-induced BPH group (3 mg/kg, 5 days/week for 2 weeks, S.C.); III: testosterone-induced BPH treated with 2ME (50 mg/kg); IV: testosterone-induced BPH treated with 2ME (100 mg/kg). (b) Densitometric quantitation of HIF-1α. (c) Densitometric quantitation of p-Smad2. Data are expressed as mean ± SD (). a or b: statistically significant from control or testosterone group, respectively, at using one-way ANOVA followed by Tukey’s post hoc test.

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