The Akt signaling and adipogenic differentiation in the absence or presence of a selective insulin growth factor-1 receptor (IGF-1R) kinase inhibitor NVP-AEW541. The protein expressions of p-Akt (Ser 473) and total Akt were detected by Western blots (a). The phase-contrast picture showed oily droplets after adipogenic differentiation of HPAs in IN and IH condition (b). The protein expressions of adipogenic markers FABP4 (c) and PPARγ (d) were measured by Western blots. The results were expressed as mean ± SEM as fold change with respect to control (IN). . Bars: mean ± SEM. and .