Cytoprotective Roles of a Novel Compound, MHY-1684, against Hyperglycemia-Induced Oxidative Stress and Mitochondrial Dysfunction in Human Cardiac Progenitor Cells

Figure 3

MHY-1684 attenuates mitochondrial ROS generation. (a) hCPCs were treated with 25 mM D-(+) glucose for 0–72 h and 1 μM MHY-1684 for 72 h. Mitochondrial ROS were labeled with MitoSOX Red, and hCPCs were analyzed by flow cytometry. (b) Histogram indicates median value ± S.E.M. of MitoSOX mean fluorescence intensity (MFI) when exposed to D-(+) glucose. MitoSOX-positive cells increased in a time-dependent manner when exposed to D-(+) glucose; however, when cotreated with MHY-1684, the MitoSOX-positive cells decreased significantly. Values are the mean ± S.E.M , as compared with the control group, as compared with the group treated with 25 mM D-(+) glucose for 72 h.

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