Procyanidins Extracted from Lotus Seedpod Ameliorate Amyloid-β-Induced Toxicity in Rat Pheochromocytoma Cells

Figure 1

Determination for both Aβ_25–35 and LSPC through measuring survival rates of cells by CCK-8. (a) Survival rate of cells under variable dosage of Aβ_25–35 with different intervene time (, , %). (b) Survival rates of cells under different dosages of LSPC with 20 μM Aβ_25–35 (black) and without Aβ_25–35 (gray) (, , %). 0 μg/mL LSPC, PC12 cells with 20 μM Aβ_25–35 group, and without Aβ_25–35; 1 μg/mL LSPC, PC12 cells with 1 μg/mL LSPC for 30 minutes before incubation with 20 μM Aβ_25–35 group, and without Aβ_25–35; 2.5 μg/mL LSPC, PC12 cells with 2.5 μg/mL LSPC for 30 minutes before incubation with 20 μM Aβ_25–35 group, and without Aβ_25–35; 5 μg/mL LSPC, PC12 cells with 5 μg/mL LSPC for 30 minutes before incubation with 20 μM Aβ_25–35 group, and without Aβ_25–35; 10 μg/mL LSPC, PC12 cells with 10 μg/mL LSPC for 30 minutes before incubation with 20 μM Aβ_25–35 group, and without Aβ_25–35; 20 μg/mL LSPC, PC12 cells with 20 μg/mL LSPC for 30 minutes before incubation with 20 μM Aβ_25–35 group and without Aβ_25–35; 40 μg/mL LSPC, PC12 cells with 40 μg/mL LSPC for 30 minutes before incubation with 20 μM Aβ_25–35 group and without Aβ_25–35; for groups vs PC12 cells without Aβ_25–35 and LSPC; for groups vs PC12 cells with Aβ_25–35 but without LSPC. All the results above are the representative of the three independent experiments.

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