Procyanidins Extracted from Lotus Seedpod Ameliorate Amyloid-β-Induced Toxicity in Rat Pheochromocytoma Cells

Figure 2

The effect of LSPC against Aβ_25–35-induced apoptosis on PC12 cells. (a–f) Cellular morphology of different groups under an electron microscope. (a) Control (200×); (b) control (400×); (c) Aβ (200×); (d) Aβ (400×); (e) LSPC (200×); (f) LSPC (400×). Control, PC12 cells; Aβ, PC12 cells with 20 μM Aβ_25–35; LSPC, PC12 cells added into 10 μg/mL LSPC 30 minutes before incubation with 20 μM Aβ_25–35. In the electron microscope, PC12 cells with 10 μg/mL LSPC and 20 μM Aβ_25–35 (e, f) showed the approximate number of cells and identical cellular morphology as a control group (a, b), while Aβ group (c, d) illustrated the reduction of cells number and abnormal morphology. (g) Hoechst staining reflects the apoptosis of cells in each group; apoptosis cells are pointed out by white arrows. (h and i) Flow cytometry calculates apoptosis rates of different groups after Annexin V/PI staining. Apoptosis rate of each group are mean ± SEM; for groups vs control; for groups vs Aβ_25–35 group. Control, PC12 cells; Aβ, PC12 cells with 20 μM Aβ_25–35; LSPC, PC12 cells added into 10 μg/mL LSPC 30 minutes before incubation with 20 μM Aβ_25–35. All the results above are the representative of the three independent experiments run in quadruplicate.

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