Research Article
Procyanidins Extracted from Lotus Seedpod Ameliorate Amyloid-β-Induced Toxicity in Rat Pheochromocytoma Cells
Figure 4
CREB/BDNF proteins expressions and mRNA expressions of intracellular BDNF in three groups (% of control, ). (a) CREB/BDNF proteins expressions by Western blotting; (b) p-CREB/CREB proteins expressions by Western blotting in (a) (each group vs control, %); (c) BDNF protein expressions by Western blotting in (a) (each group vs control, %). Control, PC cells; Aβ, PC12 cells with 20 μM Aβ25–35 group; LSPC, PC12 cells with 10 μg/mL LSPC and 20 μM Aβ25–35 group. All data are mean ± SEM. for groups vs control group; for groups vs Aβ groups. (d) mRNA expression of intracellular BDNF in each group by qRT-PCR analysis. Cells were cultured as three groups as above, including control, Aβ, and LSPC. All data are mean ± SEM. for groups vs control groups; for groups vs Aβ groups. All the results above are the representative of the three independent experiments.
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