Procyanidins Extracted from Lotus Seedpod Ameliorate Amyloid-β-Induced Toxicity in Rat Pheochromocytoma Cells

Figure 6

CREB/BDNF proteins expressions and mRNA expression of intracellular BDNF in five groups (% of control, ). (a) CREB/BDNF proteins expressions by Western blotting; (b) p-CREB/CREB proteins expressions by Western blotting in (a) (each group vs control, %); (c) BDNF protein expressions by Western blotting in (a) (each group vs control, %). Control, PC cells; LY, PC12 cells with 10 μM LY294002; LSPC + LY, PC12 cells with 10 μg/mL LSPC and 10 μM LY294002; PD, PC12 cells with 30 μM PD98059; LSPC+PD, PC12 cells with 10 μg/mL LSPC and 30 μM PD98059. All data are mean ± SEM. for groups vs control groups; for groups vs LY groups; for groups vs PD groups. (d) mRNA expression of intracellular BDNF in each group by qRT-PCR analysis. Cells were cultured as five groups as above, including control, LY, LSPC + LY, PD, and LSP + PD. All data are mean ± SEM. for groups vs control group; for groups vs LY groups; for groups vs PD groups. All the results above are the representative of the three independent experiments.

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