Degradation of TRPML1 in Neurons Reduces Neuron Survival in Transient Global Cerebral Ischemia

Figure 2

Reduction of neuron survival in OGD by TRPML1 degradation. (a) Hippocampal neuron survival rate determined by MTT assay after 3 hrs OGD in the preincubation with ML-SA1 (10 μM, ). (b) Immunoblot analysis of HA-TRPML1 expression in hippocampal neuron. (c) The survival rate of hippocampal neuron transfected with TRPML1 lentivirus determined by MTT assay after 3 hrs OGD (). β-Actin serves as a loading control. (d, f) Hippocampal neuron apoptosis was analyzed by flow cytometry 3 hrs OGD in the preincubation with ML-SA1 (10 μM, ). (e, g) The apoptosis of hippocampal neuron transfected with TRPML1 lentivirus was determined by flow cytometry after 3 hrs OGD (). All data were presented as mean ± SEM. All comparisons between groups for statistical significance were performed with one-way analysis of variance (ANOVA) with Tukey’s post hoc test. , versus Ctrl. versus OGD.

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