Aerobic Training Prevents Heatstrokes in Calsequestrin-1 Knockout Mice by Reducing Oxidative Stress

Figure 3

Structural and quantitative analysis of mitochondria. (a–c) Representative electron micrographs (longitudinal sections) of EDL fibers where small black arrows point to healthy mitochondria (see enlargements in (a) and (c)), while empty arrows point to damaged mitochondria (see enlargement in (b)). Scale bars in (a–c), 1 μm; insets, 0.1 μm. (d) Quantitative analysis of the relative fiber volume occupied by mitochondria. (e) Quantitative analysis of damaged mitochondria calculated as percentage of total. (f) Cytochrome c oxidase activity assayed in TA muscles. Data in (d) and (e) are shown as mean ± SEM; , as evaluated by two-tailed unpaired Student’s t-test for the 95% confidence intervals. Data in (f) are shown as mean ± SD; , as evaluated by one-way ANOVA followed by Tukey’s post hoc test. n = number of fibers analyzed (in panels (d) and (e)) and number of mice (in panel (f)).

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